2018-07-09

Polynucleotide phosphorylase (PNPase) is an evolutionary conserved 3',5' exoribonuclease, which plays a central role in RNA processing in bacteria and plants.

PLOS Genetics 2018, 14 (10) , e1007654. https://doi.org/10.1371/journal.pgen.1007654; George Jones. Novel Aspects of Polynucleotide Phosphorylase Function in Streptomyces. Escherichia coli polynucleotide phosphorylase (PNPase) primarily functions in RNA degradation. It is an exoribonuclease and integral component of the multienzyme RNA degradosome complex [Carpousis et al.

Polynucleotide phosphorylase

Activation. Phosphorylase a is the more active R form of glycogen phosphorylase that is derived from the phosphorylation of the less active R form, phosphorylase b with associated AMP. 2018-10-11 Polynucleotide phosphorylase (PNPase), which contains an N-terminal catalytic core and C-terminal RNA binding KH-S1 domains, is involved in RNA processing. Here we demonstrate that in Pseudomonas aeruginosa the KH-S1 domains of PNPase are required for the type III secretion system (T3SS) and bacterial virulence. Polynucleotide phosphorylase is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity. It is involved on mRNA processing and degradation in bacteria, plants, and in humans. In humans, the enzyme is encoded by the PNPT1 gene.

poly(A) polymerase; ESCHERICHIA-COLI K-12; MESSENGER-RNA; POLYNUCLEOTIDE PHOSPHORYLASE; RAPID DEGRADATION; RIBONUCLEASE-II;

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Polyguanylic acid (poly(G)) was synthesized from GDP in a yield of 60-75% by Thermus thermophilus polynucleotide phosphorylase (polyribonucleotide: orthophosphate nucleotidyltransferase, EC 2.7.7.8) at 70°C, pH 8.5 in the presence of Mg 2+.

Human polynucleotide phosphorylase (hPNPase) is an RNA-processing enzyme induced in response to type I interferons and during terminal differentiation and cellular senescence.

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Mammalian PNPase exhibits exoribonuclease and poly (A) polymerase activities, and PNPase overexpression inhibits cell growth, induces apoptosis, and stimulates proinflammatory cytokine production. In Severo Ochoa …named the enzyme he discovered polynucleotide phosphorylase. It was subsequently determined that the enzyme’s function is to degrade RNA, not synthesize it; under test-tube conditions, however, it runs its natural reaction in reverse.

Interestingly, they found that the release of mtdsRNA is dependent on Polynucleotide phosphorylase (PNPase).
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Polynucleotide Phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity. That is, it dismantles the RNA chain starting at the 3' end and working toward the 5' end. It also synthesizes long, highly heteropolymeric tails in vivo.

N62372. 0.44.

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Full=Polynucleotide phosphorylase; Short=PNPase gi|122257102|sp|Q02FT2.1|PNP_PSEAB RecName: Full=Polyribonucleotide nucleotidyltransferase;

Jul 23, 2018 Polynucleotide phosphorylase (PNPase) catalyzes the synthesis of long chain polyribonucleotides (RNA) in 5' to 3' direction from nucleotide Polynucleotide phosphorylase (PNPase), an enzyme conserved in bacteria and eukaryotic organelles, processively catalyzes the phosphorolysis of RNA, Jul 19, 2015 Purine Nucleoside Phosphorylase: Physiology, Biochemistry, and Mechanism. 1,035 views1K views. • Jul 19, 2015. 7. 0. Share. Save.